Cell Biology 9/2/99

1) Low EM of Limb Bud Mesenchyme
2) Lower level = Hypertrophied cells. They are mineralized and secreting type 10 collagen Upper cells are chondroclasts (Hylarcartilage take a columnar appearance)
3) Bottom: Bone marrow of the Epiphysis, Bone matrix of the epiphysis, zone of resting cartilage, proliferating cartilage, maturing cartilage, calcifying cartilage, ? (In respective order from bottom to top)
4) EM of the Muscle - Extracted with glycerol - see the individual myofibrils - the fibrils are lined up in register
5) Thin section of a muscle = myofibrils are composed of finer filaments
6) 200 angstroms - Clearly see the filaments that compose the myofibril. See the thick and thin filament. Thick filaments have branches that reach out to the thin filaments.
7) Myosin in solution = Kept from aggrigrating by using high salt. See the tail and one or two heads
8) Self assembly of thick filaments that form by insoluble tail regions - They are Bipolar
9) See the helical structure and the G subunits
10) Thin filaments decorated with myosin head groups vs. non-attatched
11) Neuromuscular Junction
12) Surface of the sarcolemma (They also showed a cross section of the sarcolemma, but I missed the picture )
13) Shows T tubule and sarcoplasmic recticulum
14) Depolarization signals the release the release of calcium causes conformational change. It then triggers a release of Ca.
15) Fibroheterogeneity- Some fibers stain differently
16) Satillite Cells - = A myoblast - it sits beneath the basement lamina and sarcolemma.