Cell Biology 9/2/99
| 1) Low EM of Limb Bud Mesenchyme | |
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2) Lower level = Hypertrophied cells. They are mineralized and secreting type 10 collagen Upper cells are chondroclasts (Hylarcartilage take a columnar appearance) |
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3) Bottom: Bone marrow of the Epiphysis, Bone matrix of the epiphysis, zone of resting cartilage, proliferating cartilage, maturing cartilage, calcifying cartilage, ? (In respective order from bottom to top) |
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4) EM of the Muscle - Extracted with glycerol - see the individual myofibrils - the fibrils are lined up in register |
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5) Thin section of a muscle = myofibrils are composed of finer filaments |
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6) 200 angstroms - Clearly see the filaments that compose the myofibril. See the thick and thin filament. Thick filaments have branches that reach out to the thin filaments. |
| 7) Myosin in solution = Kept from aggrigrating by using high salt. See the tail and one or two heads | |
| 8) Self assembly of thick filaments that form by insoluble tail regions - They are Bipolar | |
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9) See the helical structure and the G subunits |
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10) Thin filaments decorated with myosin head groups vs. non-attatched |
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11) Neuromuscular Junction |
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12) Surface of the sarcolemma (They also showed a cross section of the sarcolemma, but I missed the picture ) |
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13) Shows T tubule and sarcoplasmic recticulum |
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14) Depolarization signals the release the release of calcium causes conformational change. It then triggers a release of Ca. |
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15) Fibroheterogeneity- Some fibers stain differently |
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16) Satillite Cells - = A myoblast - it sits beneath the basement lamina and sarcolemma. |